Comparative examinations of tubercule bacilli in sputum by the fluorescence method, Ziehl-Neelsen stain and culture method
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چکیده
منابع مشابه
Acriflavine as a Counter-Stain in the Ziehl-Neelsen Method of Staining
a strength of 1:500 with distilled water, and the process of staining is carried out exactly in the same way as that described under the above method, except that acriflavine in the abovementioned strength is used instead of methylene blue. I use the counter-stain for half to one minute. To obtain the best results, all the detail in the careful preparation and fixation of smears, as well as the...
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Tuberculosis (TB) remains one of the leading causes of death in developing countries and its recent resurgences in both developed and developing countries warrants global attention. Globally, there were an estimated of 9.27 million incident cases of TB in 2007. This is an increase from 9.24 million cases in 2006, 8.3 million cases in 2000 and 6.6 million cases in 1990. Most of the estimated num...
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BACKGROUND & OBJECTIVES Sensitivity of Ziehl-Neelsen (ZN) method is known to be low for liquefied sputum smears. Information on the ZN sensitivity for centrifuged deposit smears is not known. This study was carried out to determine the sensitivity of ZN method for acid fast bacilli (AFB) in centrifuged deposit smears and liquefied sputum smears made from sputum samples transported in cetyl-pyri...
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To evaluate Ziehl-Neelsen (ZN) staining using variants of carbol-fuchsin solution, duplicate smears from 416 samples were stained with ZN, one set with 1% basic fuchsin and the other 0.3%. Another set of duplicate smears from 398 samples were stained with ZN, one with 1% basic fuchsin and the other 0.1%. The coded smears were read and discrepancies resolved. All samples underwent mycobacterial ...
متن کاملEvaluation of sputum staining by modified cold method and comparison with Ziehl-Neelsen and fluorochrome methods for the primary diagnosis of tuberculosis.
An improved acid-fast staining technique for sputum examination for the primary diagnosis of tuberculosis is described. The technique was modified and simplified by the elimination of heating and by combining the stages of counterstaining: making the technique easier and safer, with less risk of phenol aerosols. The efficiency of this method was evaluated by comparison with two conventional met...
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ژورنال
عنوان ژورنال: Journal of Nippon Medical School
سال: 1952
ISSN: 0048-0444,1884-0108
DOI: 10.1272/jnms1923.19.708